User manual LEICA TCS LSI BROCHURE

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Manual abstract: user guide LEICA TCS LSIBROCHURE

Detailed instructions for use are in the User's Guide.

[. . . ] A generous workspace provides extended freedom for in vivo experiments. Zoom in and out, from overview to area of interest at highest resolution and obtain fascinating results. From cell to embryo Leica TCS LSI enables you to visualize cell growth and the fascinating differentiation of cells into organs in real life from cell to embryo ­ in one system!Identify new pathways from gene to cell, from cell to animal; or examine the influence of genomic defects on the whole animal. [. . . ] Stage adapters for CO2-gas and humidification offer optimal sample conditions. Even during the experiment, remote controlled manipulators enable active specimen handling within the native environment. All accessories are offered in modular and approved kits for easy system upgrade on demand, ready for todays and future experiments. Following the changes of life Studying movements of cancer cells in bio-medical research, investigating translocations after photo-activation, studying the growth of bio films on large implants: The creativity for future experiments will come from you, the extended freedom for new research applications is provided by Leica TCS LSI. 16 Zebra fish development video 11 Confocal technology · Highest resolution · · · · · · · True confocal point scanner Spectral detector Solid state laser 405, 488, 532, 635 nm excitation AOTF controlled Fully automated Easy to use Crystal clear 3D images ­ The benefits of true spectral confocal imaging Leica TCS LSI is equipped with a true spectral confocal scanner that provides ultimate crisp resolution. Scanning the specimen in thin optical layers and detecting the fluorescence signal point-by-point results in images free from the stray light of adjacent elements. The result ­ brilliant images at very high resolution. The World's First Super Zoom Confocal Technique for new perspectives The Confocal Principle Detector Confocal pinhole Information from each signal in all optical sections is reconstructed by intelligent software into excellent 3D images, resolving the smallest detail of the specimen's structure. To ensure high quality imaging, optimal excitation is provided by up to four solid-state lasers with 488, 532 and 635 nm lines for common dyes. The broad range of applications is extended by the 405 nm laser option for nuclear staining. Maximum signal efficiency by spectral detection Maximize the signal independent from barriers posed by fixed filters and tune freely the emission band from 430 nm to 750 nm. Cross-talk of different dyes in one sample is prevented when tuning the spectral detector precisely in 10 nm steps to the respective dye. For unrivalled detection efficiency, the Leica TCS LSI uses a Beam Splitter Laser Objective Focal plane The Spectral Principle Light beam Prism Variable Spectral Detector Collimation Optics Detector 12 prism spectral detector. Minimize bleaching and cell damage by optimizing the detection range and reduce the excitation power via Acousto-Optical Tuneable Filter (AOTF). Highly innovative Zoom freely in and out, switching between overview and smallest detail without changing the objectives. The Leica Z16 APO A 16:1 super zoom offers the largest magnification range from 0, 57x to 9, 2x, whereas the Z6 APO A ranges from 0, 57x to 3. 6x ­ at excellent optical performance. The Z-zoom systems are fully apochromatic and allow to adjust magnification continuously and parallax-free. Benefit from the motorized versions Z16 APO A and Z6 APO A: Fully software controlled, the magnification can be completely altered without touching the imaging system. To achieve precise focusing for sharpest images over a wide range of 10 mm, all motorized zooms are equipped additionally with a motorized fine focus lens. Macro and Micro: New dimensions for largest specimen Benefit from the high quality macro objectives of Leica Microsystems. A novelty in confocal imaging: the tremendous working distance (WD) of 97 mm and a field of view (FOV) of 16 mm provided by the 1x apochromatic macro objective. Additionally, classic microobjectives can be adapted to use the Leica TCS LSI as a classical confocal. The standardized intuitive user interface of Leica LAS AF enables you to start your work autonomously from the first button click. Ergonomic software with workflow-oriented screens guides you through your experiment from the first confocal image up to the full 3D reconstruction of your specimen. [. . . ] Leica, the leading brand for microscopes and scientific instruments, developed from five brand names, all with a long tradition: Wild, Leitz, Reichert, Jung and Cambridge Instruments. Yet Leica symbolizes innovation as well as tradition. · Life Science Research Division Leica Microsystems' Life Science Research Division supports the imaging needs of the scientific community with advanced innovation and technical expertise for the visualization, measurement and analysis of microstructures. Our strong focus on understanding scientific applications puts Leica Microsystems' customers at the leading edge of science. Leica Microsystems ­ an international company with a strong network of customer services Australia: Austria: Belgium: Canada: Denmark: France: Germany: Italy: Japan: Korea: Netherlands: Portugal: Singapore Spain: Sweden: Switzerland: United Kingdom: USA: Barcelona Kista Heerbrugg Milton Keynes Bannockburn/lllinois North Ryde Vienna Groot Bijgaarden Herlev Rueil-Malmaison Wetzlar Milan Tokyo Seoul Rijswijk Lisbon Tel. +1 847 405 0123 Fax +61 2 9878 1055 Fax +43 1 486 80 50 30 Fax +32 2 790 98 68 Fax +1 905 762 8937 Fax +45 4454 0111 Fax +33 1 47 32 85 86 Fax +49 64 41 29 41 55 Fax +39 0257 40 3475 Fax +81 3 5421 2896 Fax +82 2 514 65 48 Fax +31 70 4132 109 Fax +852 2564 4163 Fax +351 21 385 4668 Fax +65 6773 0628 Fax +34 93 494 95 32 Fax +46 8 625 45 10 Fax +41 71 726 34 44 Fax +44 1908 609 992 Fax +1 847 405 0164 Order nos. [. . . ]

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