User manual LEICA PRECISION CRYOEMBEDDING SYSTEM BROCHURE

[. . . ] Precision Cryoembedding System Frozen Sections Become a Work of Art! Precision Cryoembedding System Easily achieve proper specimen orientation and perfect embedding with the unique Precision Cryoembedding System. Stephen Peters, to expedite and improve frozen sections and shorten the learning process for pathology residents. [. . . ] Touch the edge of a specimen to the cold base of the embedding well and gently withdraw the dispensing slide while positioning the specimen. Remove freezing block and tap the chuck stem when freezing is complete (usually 20 to 60 seconds) to remove the embedded block from the embedding well. 1 2 3 4 5 6 The Results A: Trimmed block B: Stained section on slide C: Photomicrograph A B C Frozen Block Cryoembedding Precise, On-edge Orientation Specimens are embedded and frozen in their entirety, then mapped and cut into firm, flat pieces. This technique is perfect for flimsy, tubular, curled, or angular specimens and it is particularly useful for margin resections. 1 Technique 1. Cut the embedded block into pieces on the cold cutting board, keeping pieces cold on the adjacent metal surface. (The main photo, #5 to the left, shows the central section while the inset shows a longitudinal margin. ) 6. Place the cut pieces face down (pieces are face up in the inset photo #6) in the embedding well and freeze using the procedure described under Face Down Cryoembedding. 2 3 4 5 6 The Results A: Trimmed block B: Stained section on slide C: Photomicrograph (sections repositioned in photograph) A B C Paper Cryoembedding Proper Positioning of Difficult Specimens This technique is used to maintain the orientation of delicate or flimsy specimens or to arrange multiple specimens so they will remain in the same plane for sectioning. Technique 1. Soak a small piece of lens paper in embedding medium and flatten it to the dispensing slide. Allow an end of the paper to overhang the edge of the dispensing slide. Touch the lens paper to the cold floor of the embedding well and gently pull the dispensing slide away. Trim through the paper on the trimming portion of the blade and then move to a clean, sharp portion to section the specimen. (Untrimmed block in upper portion of photo #4, trimmed block in lower portion. ) 1 2 3 4 The Results A: Large undissected specimen, dispensing slide and lens paper B: Specimen dissected with inked border C: Embedded block D: Stained sections on slide A B C D Leica Microsystems ­ the brand for outstanding products Leica Microsystems' mission is to be the world's first-choice provider of innovative solutions to our customers' needs for vision, measurement, lithography and analysis of microstructures. Leica, the leading brand for microscopes and scientific instruments, developed from five brand names, all with a long tradition: Wild, Leitz, Reichert, Jung and Cambridge Instruments. [. . . ] (06224) 143-0, Fax (06224) 143-200 We provide comprehensive systems and services for clinical histo- and cytopathology applications, biomedical research and industrial quality assurance. Our product range includes instruments, systems and consumables for tissue infiltration and embedding, microtomes and cryostats as well as automated stainers and coverslippers. Order No. [. . . ]