User manual LEICA MM NEURITE OUTGROWTH DATASHEET

[. . . ] Leica MM Neurite Outgrowth Analysis Software Drop-in for Leica MM AF · Cell-by-Cell measurement · Multiparameter analysis · Adaptive Background CorrectionTM for improved cell quantitation Leica MM AF provides a platform for neurite outgrowth experiments including both image acquisition and image analysis. [. . . ] With the flexibility of the software, researchers are able to choose whether or not to use nuclear stains. The use of nuclear stains ensures a one-to-one correlation between nuclei and cell bodies. The module includes Adaptive Background CorrectionTM, adapting the cell detection algorithm to the local intensity ranges outside the cells to provide the most robust segmentation available. This technique enables cell detection even with highly variable background fluorescence within a single image. Easy Configuration for analysis Five easy steps allow you to get started in minutes: 1. Set classification limits for outgrowths Multi-parameter analysis The application module can generate a number of field or cell-by-cell parameters. Field measurements include: Measurement options include: · Number of cells · Total outgrowth · Mean outgrowth per cell · Total processes · Mean processes per cell · Total branches · Mean branches per cell · Total cell body area · Mean cell body area · Straightness of processes · Cells exhibiting significant growth · Percentage of cells exhibiting significant growth Interactive data display Image acquisition Image acquisition with Leica MM AF. [. . . ] All the filaments and cell bodies are then measured. Data export for further analysis www. leica-microsystems. com [. . . ]