User manual LEICA MM MULTI WAVELENGTH CELL SCORING DATASHEET

[. . . ] Leica MM Multi Wavelength Cell Scoring powered by MetaMorph® Analysis Software Drop-in for Leica MM AF · Multi-Parameter analysis of up to seven wavelengths · Identification of subpopulations of cells · Adaptive background correctionTM for improved segmentation · Field and Cell-by-Cell measurements The Leica MM Multi Wavelength Cell Scoring Application Module for Leica MM AF software is designed for the scoring of cells with up to seven fluorescent stains. [. . . ] This technique enables probe detection even with highly variable background fluorescence within a single image. The simple interface minimizes setup efforts and at the same time enables users to customize the settings and measurements to obtain the best possible results specific to the type of cells used in the experiments. Multiple wavelengths Leica MM AF provides a flexible integrated solution for multi wavelength image acquisition and analysis. Blue: Hoechst 33342, red: AntiPhospho-Histone H3 (Ser28), green: Transfluor® vesicles. Configuration for analysis Set the number of wavelengths used and repeat the following steps for each wavelength: 1. Specify approximate minimum and maximum widths of the objects to be detected 4. Adjust detection sensitivity by specifying the intensity above local background 5. Optionally specify the reporting parameters Multi-parameter analysis Field measurements include: · Wavelength-specific count and percentage of negative and positive cells · Scoring profile counts Cell-by-cell measurements include: · Wavelength-specific stained area, and integrated and average intensities · Scoring profile Powerful data export capabilities Interactive data display Accurate segmentation Detection of cells uses Adaptive Background Correction and displays interactive graphics on the original images indicating scoring for each wavelength for immediate verification of accuracy. [. . . ] All settings can be saved for future experiments. www. leica-microsystems. com [. . . ]