User manual LEICA MM LIVE-DEAD DATASHEET

[. . . ] Leica MM Live/Dead Analysis Software Drop-in for Leica MM AF · Precise cell staining quantitation · Generate area and intensity measurements · Adaptive Background CorrectionTM for improved cell quantitation · Field and Cell-by-Cell data logging The Leica MM Live Dead Application Module for the Leica MM AF software is designed to automate accurate identification of cells after staining with a variety of live/dead staining kits. [. . . ] It can also be used with exclusive markers to detect two different populations. powered by MetaMorph® The module utilizes Adaptive Background Correction adapting the cell detection algorithm to the local intensity ranges between and within cells to provide the most robust segmentation available. This technique enables probe detection even with highly variable background fluorescence within a single image. The simple interface minimizes setup efforts and at the same time enables users to customize the settings and measurements to obtain the best possible results specific to the type of cells used in the experiments. Easy Configuration for analysis Five easy steps allow you to get started in minutes: 1. Optionally choose the parameters for reporting Multi-parameter analysis Choose the measurements to capture, including: · Live, dead and total cell counts · Percentage of live and dead cells · Per-wavelength average measurements · Per-wavelength per-cell measurements Powerful data export capabilities All measurements can be directly exported to a text file or Microsoft® Excel® for further analysis. "MetaMorph® is a Registered Trademark of MDS Analytical Technologies" Image acquisition CHO cells. Cells were incubated for ~24 hours and apoptosis was induced by incubating different concentrations of Staurosporine for 6 hours. [. . . ] The modules can be incorporated into a Leica MM AF journal to increase the customization and automation of your analysis. Cell segmentation and analysis The Live Dead module identifies live and dead cells simultaneously Data exporting Measurements can be exported directly to a spreadsheet program for further analysis. www. leica-microsystems. com [. . . ]