User manual LEICA MM GRANULARITY DATASHEET

[. . . ] Leica MM Granularity Analysis Software Drop-in for Leica MM AF · Counts nuclei and punctate · Adaptive Background CorrectionTM for improved cell quantitation · Choice of six granularity indices · Field and Cell-by-Cell data logging The Granularity Application Module for the Leica MM AF software from Leica Microsystems is designed to facilitate the segmentation and quantitation of punctate. [. . . ] A simple interface minimizes setup efforts and at the same time enables users to customize the settings and measurements to obtain the best possible results specific to the type of cells used in the experiments. Configuration for analysis Multi-parameter analysis The Application Module can generate a number of field or cell-by-cell parameters, including: · Granule and Nuclear Count · Granule and Nuclear Total and Mean Area · Granule and Nuclear Integrated and Average Intensity The module can determine and log the following six granularity indexes: · Texture Index · Cellular Texture Index · Gradient Index · Cellular Gradient Index · Laplacian Index · Cellular Laplacian Index Adaptive Background CorrectionTM for improved segmentation Using standard thresholding can result in incorrect grain counts (orange), Adaptive Background Correction improves grain quantitation even in cells with high background (red arrow). Assay kit: Molecular Probes, Vybrant Assay Kit #7. Step 1. Specify the size range of punctate and intensity above local background. If using a nuclear stain, select the nuclear image, size range of nuclei and intensity above local background. Optionally set reporting parameters Interactive data display Once the analysis is run, the Cellular Results table allows you to interactively view individual cells' data. Clicking one or multiple cells in the image highlights the data for the selected cell(s) in the table. Powerful data export capabilities Pits (white) and nuclei (green) can be simultaneously segmented, saving analysis time. Grains are accurately identified even in cells with high background (red arrow). Customization through journaling Journals are sophisticated and powerful macros that record and perform a series of tasks without the need for a programming language. [. . . ] "MetaMorph® is a Registered Trademark of MDS Analytical Technologies" www. leica-microsystems. com [. . . ]