User manual LEICA MM CELL HEALTH DATASHEET

[. . . ] Leica MM Cell Health Analysis Software Drop-in for Leica MM AF · Analysis of three fluorescent probes · Adaptive Background CorrectionTM for improved segmentation · Field and Cell-by-Cell data logging In some diseases, such as cancer, mutations may cause apoptotic pathways to malfunction, allowing uncontrolled proliferation of tumorigenic cells. [. . . ] The simple interface minimizes setup efforts and at the same time enables users to customize the settings and measurements to obtain the best possible results specific to the type of cells used in the experiments. Configuration for Analysis 1. Optionally choose the reporting parameters Multi-parameter analysis The Application Module can generate a broad range of parameters, including: · Counts of viable, early and late apoptotic, and necrotic cells · Percent of viable, early and late apoptotic and necrotic cells · Area, integrated and average intensity of nuclei Powerful data export capabilities All measurements can be directly exported to a text file or Microsoft® Excel® for further analysis. "MetaMorph® is a Registered Trademark of MDS Analytical Technologies" Interactive data display Multiple probes acquisition Adherent Chinese Hamster Ovary (CHO-K1) cells were incubated with various concentrations of staurosporine. From top to bottom: control, 0. 1 M staurosporine, and 3 M staurosporine. BIOMOL International's Mit-E- Mitochondrial Permeability Detection (Hoechst 33342 + JC-1). Courtesy of BIOMOL International. Once the analysis is run, the Cellular Results table allows you to interactively view individual cells' data. [. . . ] Green: viable, blue: early apoptotic, purple: late apoptotic, red: necrotic. Data export for further analysis www. leica-microsystems. com [. . . ]