User manual LEICA MM CELL CYCLE DATASHEET

[. . . ] Leica MM Cell Cycle Analysis Software Drop-in for Leica MM AF · Quantitation of cell cycle stages · Adaptive Background CorrectionTM for improved segmentation · Field and Cell-by-Cell data logging Cells have multiple checkpoints that can impede progression past the stages of G0/G1, G2 or the midpoint of M during the cell cycle. [. . . ] Additionally, a mitosis-specific probe may be used to better identify M-phase cells and an apoptosis-specific probe may be used to identify cells undergoing apoptosis. powered by MetaMorph® Images can be acquired using one, two or three different wavelengths for the DNA stain, M-phase marker and apoptosis marker. The module utilizes Adaptive Background Correction adapting the cell detection algorithm to the local intensity ranges between and within cells to provide the most robust segmentation available. This technique enables probe detection even with highly variable background fluorescence within a single image. A simple interface minimizes setup efforts and at the same time enables users to customize the settings and measurements to obtain the best possible results specific to the type of cells used in the experiments. Configuration for analysis Step 1. Set the classification criterion for mitosis by specifying the intensity of the DNA stain or an optional mitotic-specific stain. If using an apoptotic stain, select the stain area, size range of cells and intensity above local background. Preview classification results and interactively adjust cutoff values. Optionally set reporting parameters. Multi-parameter analysis The Application Module can generate a number of field or cell-by-cell parameters, including: · Count and percentage of G0/G1, S, G2, early M, late M and apoptotic cells · DNA area, mitotic and apoptotic integrated and average intensities "MetaMorph® is a Registered Trademark of MDS Analytical Technologies" Multiple wavelength acquisition The software acquires multiple wavelengths and color combines the images during visualization (right). Interactive data display Once the analysis is run, the Cellular Results table allows you to interactively view individual cells' data. [. . . ] The modules can be incorporated into a Leica MM AF journal to increase the customization and automation of your analysis. Powerful data export capabilities All measurements can be directly exported to a text file or Microsoft® Excel® for further analysis. Interactive graphs Cell classifications can be set by interactively moving cutoff values directly on the graphs. www. leica-microsystems. com [. . . ]