User manual LEICA MACROFLUO

[. . . ] Leica MacroFluoTM User manual Dear User, Thank you for your faith in our product. Leica MacroFluoTM with zoom 6. 3:1 or 16:1 are worldwide the only macro documentation systems for fluorescence. The MacroFluoTM concept combines the advantages of macroscopy ­ large fields of view, large working distances, parallax-free and precise imaging ­ with fluorescence technology (including multi color fluorescence) at a high resolution. For this purpose, we combined the excellent optics of the apochromatic zoom systems Leica Z6 APO (6. 3:1 Zoom) or Z16 APO (16:1 Zoom) with the brilliant Leica fluorescence technology. [. . . ] Tighten the fine focusing (4. 5) with 3 hexagonhead screws (4. 7) to the zoom system (4. 2). Fasten planapochromatic objective (4. 3) counterclockwise on the fine focusing (4. 5). 14 Leica MacroFluo ­ Assembly 2. 8 UV protection screen Fasten UV protection screen (5. 5) with clamp (5. 4) on the hinged arm (5. 1). Fasten hinged arm with UV protection screen to the carrier using the clamp (5. 2). 3 The hinged arm can be opened and closed via the central rotary knob (5. 3). In the opened state, all joints are freely movable and the hinged arm can be placed in any position. 5 4 2 1 5 Hinged arm with UV protection screen Always position the UV protection screen so that the operator can ever look directly onto the light spot (see p. 9). 1 Hinged arm 2 Clamp with clamping screw for fastening to carrier 3 Central rotary knob 4 Clamp with clamping screw for fastening to UV protection screen 5 UV protection screen Leica MacroFluo ­ Assembly 15 2. 9 Lamp housing The Leica lamp housing 106 z (11 504 069) is the light source for the incident illuminator LRF 4/22. It is used with 100-W mercury burners (11 500 138) or 103 W/2 (11 500 321) with extended life, the supply unit ebq 100 W and 103 W/2 (11 500 325) and straylight protection (10 446 271). 2. 10 Instrumentation of nosepiece disc The nosepiece disc can be fitted with a total of 4 filter blocks (filter system). 25) are available for various macro and multicolor applications (additional filter blocks upon request). The filter block offer from Leica Microsystems is continuously expanded with respect to the latest application requirements. The filter blocks (filter systems) consist of a combination of excitation filter, beam-splitting mirror and suppression filter. Please read ­ the separate operating instructions M2-216-1 and the safety instructions for the Leica lamp housing ­ the operating instructions and safety instructions for the supply unit ebq 100 ­ the operating instructions and safety instructions of the burner manufacturer and specifically its notes in reference to lamp breakage and the release of mercury. Fasten the stray-light protection to the lamp housing 106 z (1. 9) as described in the operating instructions M2-216-1. Fasten the lamp housing 106 z (1. 9) to the adapter component of the incident illuminator as described in the operating instructions M2-216-1. Connect the cable from the lamp housing to the supply unit ebq 100 W and 103 W/2. Insert the 100-W or 103 W/2 mercury burner as described in the operating instructions M2-216-1 in the lamp mount and in the lamp housing. It is not necessary to occupy all 4 positions. Remove the cover (6. 1) on the right side of the illuminator by pulling it up (recess [6. 2] for placing a finger on the bottom of the cover). Push the filter block with the tapered end of the dovetail guide (7. 1) first into the guide until it stops. Turn the nosepiece disc (6. 3) and populate the 4 positions. Only switch on the supply unit after you familiarized yourself with the functions described in the "Operation" chapter of the Leica MacroFluoTM system in transmitted light. After activation, the burner must be adjusted immediately (see operating instructions M2-216-1). 16 Leica MacroFluo ­ Assembly The 4 positions of the nosepiece disc are each labeled with the numbers 1 through 4 on the left side of the dovetail guide. In addition, the active position in the beam path is displayed on the outside of the nosepiece disc (6. 3). The filter blocks are supplied with self-adhesive labels numbered 1 through 4 and labels with short designations of the filter block (e. g. Affix the label 1 2 3 4 in the rectangular recess at the front of the illuminator (Fig. Compare which position on the nosepiece disc (number on the left of the dovetail guide) you equipped with which filter block. [. . . ] These filter blocks contain the excitation filter, dichromatic beam-splitting mirror and suppression filter. ­ The excitation filter selects exactly those wavelengths from the beam selection of the light source that are used for exciting the fluorescence. This is made possible by highest transmissivity in the excitation range, while using the highest possible suppression effect in other wavelength ranges. ­ The dichromatic beam-splitting mirror reflects the short-wave excitation radiation to the specimen, while being transparent for the fluorescent light emitted from the specimen. [. . . ]