User manual LEICA CV5030

[. . . ] Leica CV5030 Robotic Coverslipper Operating Manual Leica CV5030 V2. 1 English - 08/2008 Always keep this manual near the instrument. Read it carefully prior to operating the instrument! NOTE The information, numerical data, notes and value judgments contained in this documentation represent the current state of scientific knowledge and state-of-the-art technology as we understand it following thorough investigation in this field. We are under no obligation to update the present manual periodically and on an ongoing basis according to the latest technical developments, nor to provide our customers with additional copies, updates etc. contained in this manual we exclude liability as far as permissible according to the national legal system applicable in each individual case. [. . . ] Adjustment options for the stroke length: In increments of 10 from: 0 = no change -100 = reduction by 10 mm to Label field -1mm Adjusting the correction value by "-10" results in a reduction of the stroke length by 1 mm. 6 mm, the reduction of the stroke is measured from the label field (Fig. 35). The application quantity is not changed, but only the length of the coverslip mountant stroke. Example: For a size 40 cover slip, the normal stroke length is 28 mm. The setting "-10" means a reduction of the stroke length by 1mm. Operation 5. 4. 3 Setting menu B parameters Coverslip Position Corr -- correction of the cover slip placement position Here, you can adjust or modify the position at which the cover slip is placed on the specimen slide. Adjustment options for the cover slip position: In increments of 5 from: -30 = -3. 0mm to +30 = +3. 0 mm 30 25 20 15 10 5 0 0 2 mm Label field Placement direction = +3 . 0 mm = +2. 5 mm = +2 . 0 mm = +1. 5 mm = +1 . 0 mm = +0. 5 mm = 0. 0 mm -30 -25 -20 -15 -10 -5 = = = = = = -3 . 0 mm -2. 5 mm -2 . 0 mm -1. 5 mm -1 . 0 mm -0. 5 mm Fig. 36 Positive values mean a shift of the placement point towards the label field. The value "0" corresponds to a distance of approx. Speaker Volume Level Adjusts the volume of the audible signals (button sounds and alarms). Adjustment options for the volume: In increments of 1 from 1 (quiet) --> 3 (loud). The alarm sounds and audible signals of the buttons cannot be disabled completely. Leica CV5030 ­ Robotic Coverslipper 35 5. Operation Stroke Position Corr -- start position correction of the coverslipping mountant Here, you can configure a correction for the position at which the application stroke of the coverslipping mountant on the specimen slide is to begin. 80 = +8 mm 90 = +9 mm 100 = +10 mm Label field Stroke direction The value `0' corresponds to a distance of approx. The coverslipping mountant is applied toward the label field. 0 6mm Fig. 37 Positive values mean a shift of the starting point towards the label field. Coverslip Limit Adjust -- adjusting the limit value for coverslips Here, you can configure the limit value for the COVERSLIPS LOW display. Adjustment procedure: place a cover slip stack of the desired height (approx. The transfer module moves to the cover slip magazine and lowers itself down to the top cover slip. If this value is reached during coverslipping, the message COVERSLIPS LOW is displayed. The cover slip magazine should be refilled. The setting of the limit value for the COVERSLIPS LOW display is a universal value that is valid for all parameter sets (SET 1 through SET 4). 36 Operating Manual V 2. 1 ­ 08/2008 5. Operation Exiting the configuration menu · When all values have been checked and configured, press the STANDBY button to exit the configuration menu. · The display shows READY along with the most recently edited parameter set. · To switch the parameter set, follow the procedure outlined in Chap. 32). 5. 4. 4 Parameter settings for various cover slip mountants The table on the following pages is a compilation of parameter settings for coverslipping mountants from various manufacturers. These values are merely a recommendation that can be affected by many factors at the location of the instrument. The data listed in the table were measured under the following conditions: Wet coverslipping, bath liquid: xylene Room temperature: 25 °C Section thickness of the specimens: approx. 3 m Cover slip size: 50 mm "Mountant Stroke" setting: 50 As a result of different location and working conditions, such as higher room temperature, great height or extreme humidity, the values specified here may not provide the desired results and have to be optimized. Leica CV5030 ­ Robotic Coverslipper 37 5. Operation Parameter settings for various coverslipping mountants (continued) The information in the "Correction values" column is valid for the following settings: LEN (menu A, STROKE LENGTH CORR) = Correction of the stroke length of the coverslipping mountant. CORR) = Correction of the start position of the coverslipping mountant. Brand name Xylene-based: Entellan NEW DPX DPX Vitro-Clud Permount Histofluid CV Mount Quick-D Consul-Mount Hico-Mic Pertex DePex Manufacturer Mountant Mountant type volume Nozzle size Correction values Merck BDH Laboratory Supplies England Merck R. [. . . ] 61 Cover with cutout, for loading bath, only together with bath insert for Leica specimen slide holder for 30 specimen slides - 0478 39593 Order No. 14 0478 40337 Schott bottle with cover, bottle for coverslipping mountant 250 ml, empty, with cover Order No. Optional Accessories Cover slip magazine, cover slip magazine multi-size TM with inserts for holding various common cover slip sizes 40-60 x 22 mm 40-60 x 24 mm Fig. 14 0478 39749 Exhaust hose, solvent resistant, flexible, 3 m length, diameter 32 mm Order No. [. . . ]