User manual LEICA CM3050 S

[. . . ] Cryostat Instruction Manual Leica CM3050 S ­ Cryostat English V1. 5 ­ 03/2010 Always keep this manual near the instrument. Read carefully prior to operating the instrument. Important note The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and stateof-the-art technology as we understand it following thorough investigation in this field. We are under no obligation to update the present manual periodically and on an ongoing basis according to the latest technical developments, nor to provide our customers with additional copies, updates etc. contained in this manual we exclude liability as far as permissible according to the national legal system applicable in each individual case. [. . . ] Once the preselected number of sections has been carried out (= section counter reading `0'), the specimen head will stop automatically in the lower limit position. the value from which the preset counter counts downward) can be selected in the corresponding menu item. In order to be able to set a reference value, the preset counter function must be active. If the preset counter is deactivated (see diagrams above), press to activate. 30 Instruction Manual V1. 5 - 03/2010 5. Quick-freezing at maximum specimen temperature* (instruments with specimen cooling only) Press key until menu item `Fast object freezing' is displayed: F AST OBJ FREEZ IN OFF OBJ FREEZ IN ON The automatic defrost cycle takes place once every 24 hours at the selected defrost time. Duration of defrost cycle: Press key until menu item `Defrost period' is displayed: DEFROST PE R I OD C HAMB E R : 08 MIN or F AST · Use defrost keys ( or ) to adjust defrost time. (Only if necessary - see below). Important information for setting the defrost parameters Starting time of defrost cycle: We recommend to program the automatic defrost cycle for a time outside regular working hours as a period of 4 hours should pass after the defrost cycle before work with the instrument is resumed (see also chapter 5. 7. 1 `Automatic chamber defrost cycle'). Defrost cycle duration: The duration of the chamber defrost cycle is set to 8 minutes in factory. This setting should only be changed, if the evaporator is not completely de-iced during the 8-minute cycle. In such cases, increase the defrost cycle duration gradually until the the evaporator is ice-free after defrosting. The selected defrost cycle duration is valid for both automatic and manual chamber defrost cycle. Press lock/unlock key to activate or deactivate (ON/OFF). Real time Press key until menu item `Time' is displayed: T IME 10 : 23 or ) to set real time. · Use arrow keys ( Setting minutes as well as hours in 1-minute steps. Starting time of automatic defrost cycle: Press key until menu item `Defrost time' is displayed: DEFR T IME 4 : 30 (Time on display = start time of defrost cycle) · Use arrow keys ( or sired defrost start time. ) to select the de- *) with 90° prism - see also chapter 5. 5. 2. Leica CM3050 S ­ Cryostat 31 5. Operation 5. 3. 5 Status display Each individual parameter is displayed in a certain area of the display screen. Upper line: In the menu (see chapter 5. 3. 4): indication of selected menu item. Standard indication: Chamber temperature (instruments with specimen cooling: chamber and specimen temperature) Standard indication upper line: Instruments with chamber and specimen cooling Instruments with chamber cooling only CT-30°C HOME OT - 35°C CT-30°C HOME - Upper line Lower line 1 1 2 2 3 3 4 4 5 5 6 6 7 7 8 8 9 9 10 11 12 13 14 15 16 10 11 12 13 14 15 16 Digits 1 and 2 indicate sectioning stroke position: · · · Sectioning stroke (outside sectioning window) Retraction stroke Sectioning stroke (inside sectioning window) Sectioning stroke Digits 3 - 6 indicate coarse feed position: · Rear limit position: · Front limit position: Indication of `malfunction' or `handwheel locked': · Microtome plug loose, implausible values etc. : · Handwheel is locked: HOME STOP Digits 7 - 16 indicate the following parameters: · Refrigeration status: Digit 15 = cryochamber Digit 16 = specimen Meaning of Refrigeration OFF refrigeration symbols: Refrigeration ON Defrosting active · Section thickness (set value) · Section counter (actual value) · Indication of total section thickness (actual value) · Preset counter Digits 7 - 16: Scroll via arrow buttons to display individual parameters : [ ]: ???? LOCKED Sectioning window [ ] Indication `LOCKED' completely overlays digits 3 through 16, i. e. while `LOCKED' is displayed, no other parameters will be displayed in digits 3 - 16 of the lower line. · Further indications: see chapter 6. 1 `Error messages indicated in the display screen' Retraction stroke 32 Instruction Manual V1. 5 - 03/2010 5. Operation 5. 3. 6 Indication of refrigeration state, section thickness setting and counters These parameters can be displayed via the arrow keys. , the parameters are disWhen pressing played in the sequence listed on this page; , they are displayed in rewhen pressing verse order. after the last parameter the display will automatically start again with the first. Refrigeration state CT-30°C HOME OT - 35°C 4. Total section thickness counter CT-30°C OT - 35°C µ 0 0 0 0 . [. . . ] 14 0201 39124 Ovewr-disc heat extractor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 0201 39125 Bin for discs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 0201 39127 Case w/embedding systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 0712 40151 Easy Dip staining cnt. grn. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [. . . ]