User manual LEICA CM1950

[. . . ] Cryostat Operating Manual Leica CM1950 , Rev C, English, 1v3 ­ 04/2010 Always keep this manual near the instrument. NOTE The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and stateof-the-art technology as we understand it following thorough investigation in this field. We are under no obligation to update the present manual periodically and on an ongoing basis according to the latest technical developments, nor to provide our customers with additional copies, updates etc. contained in this manual we exclude liability as far as permissible according to the national legal system applicable in each individual case. [. . . ] The mechanical handwheel brake is nabled when the yellow LED is lit in the M-STOP field. Hold the button to section at maximum speed. Release the button to continue sectioning at the previously selected speed (see controller, above). Switch from slow speed range to fast speed range: while switching on the instrument, press and hold down the Vmax button. 34 Speed ranges Slow: 0-50 strokes/min Fast: 0-85 strokes/min Vmax: 85-90 strokes/min The handwheel must also be locked when working on the specimen head. Press to brake the handwheel electronically (LED lit) ­ specimen stops in lower (6 'clock) position. - Specimen head stops at bottom (for BRAKE, automatic electronic braking takes place). For motorized sectioning, does not have to be unlocked; continue working by pressing both the RUN/STOP and ENABLE keys. If, during manual work using handwheel, you have braked using BRAKE also use BRAKE to release! Sectioning modes The instrument can be used in manual and motorized modes. The following settings are available: · Single stroke (SINGLE) or continuous stroke (CONT. ) in automatic mode, as well as · ROCK (sectioning using handwheel) in manual mode. When switching the instrument on, no operating mode is active for safety reasons. 34 Operating Manual, Rev C, 1v3 ­ 04/2010 7. Instrument Controls Disinfection Duration - 30min Duration - 180 min UVC key - to activate / deactivate the disinfection cycle and/or to acknowledge interruption of a disinfection cycle. mode For current information about certificates and recommendations, visit: www. leica-microsystems. com/downloads/Histology Systems/Certificates Fig. 35 UVC disinfection is effective when disinfecting surfaces and air within the irradiated working area of the cryostats Leica CM1850UV, CM1900UV and CM1950 at -20°C (Table 1). For powerful disinfection, we recommend irradiation for three hours (CM1850UV/CM1950) and four hours (CM1900UV). Vegetative bacteria including Mycobacterium tuberculosis, bacterial endospores (Bacillus sp. ) and fungi are killed during this time. Viruses, including such resistant species as, for example, hepatitis viruses, are also deactivated to at least 4 log10 units (99. 99 %). Medium disinfection can be attained through short irradiation for 30 minutes (CM1850UV/CM1950) and 40 minutes (CM1900UV). This reduces vegetative bacteria including Mycobacterium tuberculosis and sensitive viruses such as the influenza A virus (also including the highly pathogenic avian influenza virus H5N1) and the poliovirus by at least 5 log10 units (99. 999%). UVC irradiation within the working area of the cryostats can provide reliable and efficient disinfection of surfaces and the air and significantly reduces the risk of infection. We recommend wiping off visible contamination in the cryostat with an alcohol-based disinfectant prior to using the UV lamps. The germicidal effect of the irradiation is restricted to the directly irradiated areas, which is why UVC irradiation cannot be a replacement for regular chemical disinfection of the cryostat chamber. Specimens and section waste must be thoroughly removed from the cryochamber first (e. using the optional extraction system, or a paper towel soaked with Cryofect or alcohol-based disinfectant). Before UV disinfection, move the anti-roll guide to the side to allow complete disinfection. [. . . ] · Lightly tilt the fluorescent tube down to the right and pull it out of the clip (3). · Hold the lamp with both hands and pull it to the right out of the holder (4). Fig. 74 3 4 Only use lamps of the same specification! Installation of the new lamp Fig. 75 Type: OSRAM DULUX L 18W/ 840. · Hold the lamp in the correct mounting position as shown and push it to the right until it engages in the holder. 72 Operating Manual, Rev C, 1v3 ­ 04/2010 13. [. . . ]